The Wagner Lab is interested in genetically engineering cells to fight infectious diseases. Cell and gene therapy is powerful new technology and a completely novel class of medical therapy. Lymphocytes engineered to express chimeric antigen receptors (CAR T cells) have radically improved survival for certain leukemias, which lead to the first FDA-approved genetically engineered cell therapy (2017). As a result, cell and gene therapy has become an active and exciting field of research. The Wagner Lab is leveraging this technology and adapting it to fight HIV and other serious infectious diseases. Under supervision of the PI and other research scientists, the successful candidate would learn the required laboratory methods to genetically engineer T cells, which includes methods to assemble genetic constructs, introduce new chimeric genes into cells, and disrupt unwanted genes using methods such as CRISPR. The succesful candidate will also analyze these cells in vitro, and test them in in vivo models. The succesful candidate would be part of a small team collaboratoring on several larger projects, would be resposnible for conducting their own experiments, and would be encouraged to inovate and create solutions to many scientific and engineering challenges. If lab work is successful there are opportunities to present data at international meetings and play a role in authorship of manuscripts and patents.
This is a unique type of position that requires significant individual initiative and motivation to learn and understand both existing and new scientific methods and knowledge. The Research Scientist will be given the opportunity and be responsible for conducting independent and creative research related to the overall mission of the laboratory. This is an ideal position for those considering graduate school in a related field or medical school in the near future (up toup to two years).
Overall responsibilities include acquiring an in-depth understanding of the field in which they are working, and working with the PI, as well as independently, on the planning, design, execution, trouble-shooting, and interpretation of experiments; makes detailed observations, records data and presents results; assists post docs and fellows in the day-to-day activities and interprets experiments related to assigned projects. The Research Scientist will spend the majority of their time making contributions to on-going laboratory projects, requiring the ability to use independent judgment to address tasks and projects, and will identify and refine existing procedures and practices. The incumbent may also participate in the management of a small rodent colony.
Seattle Children’s Employer Highlights
- Bachelor’s Degree in biology, microbiology, immunology, molecular biology or biochemistry.
- Prior experience in a laboratory setting.
- Research experience in biochemistry, molecular biology, and tissue culture techniques is highly preferred.