Research Associate II - Bench

Job ID 2020-35446
Area of Interest
Research & Development
Location Name
307 Westlake
FTE/Hours per pay period
1.0 FTE (80 hours per bi-weekly pay periods)
Work Status
Regular
Department
Global Infectious Disease
Shift
Day Shift
Primary Location : City
Seattle
Primary Location : State/Province
WA

About Us

Hope. Care. Cure. These three simple words capture what we do at Seattle Children’s – to help every child live the healthiest and most fulfilling life possible.  Are you ready to engage with a mission-driven organization that is life-changing to many, and touches the hearts of all? #HOPECARECURE

 

As one of the nation's top five pediatric research centers, Seattle Children's Research Institute is dedicated to providing hope, care, and cures to help every child live the healthiest and most fulfilling life possible.

 

Our investigators are involved in hundreds of projects that cover every phase of research, from studying how diseases work to improving investigational therapies. They have pioneered groundbreaking cystic fibrosis treatments and cutting-edge cancer therapies that help a child's immune system defeat cancer, and made other major contributions to pediatric medicine. 

 

Researchers work in close collaboration with one another, their colleagues at partner institutions including the University of Washington and Fred Hutch and our healthcare providers at Seattle Children's Hospital, one of U.S. News & World Report’s top children's hospitals. This collaboration is one of our key strengths, allowing our faculty to draw on a variety of disciplines and techniques as they pursue solutions to some of medicine's most complex problems.

 

We are committed to not only treating disease but to eliminating it. Help us achieve our vision of being a worldwide leader in pediatric research aimed to improve the health and well-being of children. If you are interested in a challenging career aimed at groundbreaking research, Seattle Children's Research Institute is the place for you.

Overview

Urdahl Lab – Tuberculosis

 

Despite widescale global use of BCG as a vaccine against Mycobacterium tuberculosis, the agent that causes tuberculosis (TB), TB continues to be one of the world’s biggest infectious killers. A new and effective vaccine is urgently needed, but fundamental insights into how protective immunity is best achieved is needed to rationally design such a vaccine. The Urdahl Lab at the Center for Global Infectious Disease Research at Seattle Children’s Research Institute is particularly interested in understanding the nature of protective immunity against tuberculosis and the barriers that impede these protective responses from being established. They are actively recruiting for a Research Associate to support their team.

 

The successful applicant will be integrated into ongoing projects in the lab and will conduct and analyze experiments within a TB Biohazard Safety Level III lab, as well as provide general support of the lab. Enthusiasm, curiosity, and initiative to learn new methods and perform work efficiently and carefully are essential. Training in techniques that are unfamiliar will be provided. This position requires organization skills needed to maintain a detailed lab notebook and multi-task in a fast-paced environment.

Candidate should have a bachelor’s degree in Microbiology, Biochemistry, Immunology, or a related field. Scientists must have at least 5 years of research experience. The Urdahl lab values laboratory experience in immunology, microbiology, and working with animals.

 

Key responsibilities:


-Perform standard and advanced laboratory procedures using molecular, cellular, and tissue culture techniques, some of which will be in a TB Biohazard III level laboratory:
-Conduct animal experiments (tissue harvests, cell preps, and animal infections) as directed by PI/Research tem members in accordance with IACUC protocol(s)
-Prepare samples for RNA extraction/library preparation for both single cell and bulk RNA sequencing
-Oversee management of mouse colony and participate in genotyping and weaning.
-Perform flow cytometric analysis

-Work in collaboration with team members to execute experimental protocols:
-Assist in designing experiments when appropriate
-Meet with team members to discuss experimental results
-Attend and present at lab meetings
-Perform data documentation, processing and analysis:
-Provide support for postdoctoral fellows and graduate students as needed
-Develop and maintain databases; ensure information is entered in a timely manner, is accurate and complete; ensure data collection is standardized; ensure data is maintained in a manner suitable for analysis and evaluation

 

The following are required:


-Experience in laboratory research
-Excellent communication skills
-Excellent organizational skills
-Knowledge of MS Office tools such as Word, Excel, Power Point
-Experience with harvesting mouse tissues and handling live animals, or at least an interest in doing so.
-Familiarity with the at least some of the following techniques:
-Cell culture
-DNA/RNA isolation
-Flow cytometry
-PCR

 

Requirements

Required Education/Experience:
- Bachelor’s Degree in Microbiology, Biochemistry, Immunology, or related field
- At least five (5) years laboratory experience.

Required Credentials:
- N/A

Preferred:
- Master’s Degree in Microbiology, Biochemistry, Immunology, or related field
- Previous research experience in biochemistry, molecular biology, and tissue culture techniques.

 

 

Our Commitment to Diversity

Our community welcomes diverse experiences, backgrounds, and thoughts as this is what drives our spirit of inquiry and allows us to better connect with our increasingly diverse patients and families. Our organization recruits, employs, trains, compensates, and promotes without regard to race, religion, color, national origin, gender (including pregnancy, childbirth, or related medical conditions), sexual orientation, gender identity, gender expression, age, status as a protected veteran, status as an individual with a disability, or other applicable legally protected characteristics.

 

The people who work at Seattle Children’s are members of a community that seeks to respect and celebrate all the qualities that make each of us unique. Each of us is empowered to be ourselves within this community, which cultivates and promotes equity, diversity, and inclusion at all levels.

 

Seattle Children’s is proud to be an Equal Opportunity Workplace and Affirmative Action Employer.

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