Research Scientist II

Job ID 2020-35762
Area of Interest
Research & Development
Location Name
1920 Terry
FTE/Hours per pay period
1.0 FTE (80 hours per bi-weekly pay periods)
Work Status
Immunotherapy Integration Hub
Day Shift
Primary Location : City
Primary Location : State/Province

About Us

Hope. Care. Cure. These three simple words capture what we do at Seattle Children’s – to help every child live the healthiest and most fulfilling life possible.  Are you ready to engage with a mission-driven organization that is life-changing to many, and touches the hearts of all? #HOPECARECURE


As one of the nation's top five pediatric research centers, Seattle Children's Research Institute is dedicated to providing hope, care, and cures to help every child live the healthiest and most fulfilling life possible.


Our investigators are involved in hundreds of projects that cover every phase of research, from studying how diseases work to improving investigational therapies. They have pioneered groundbreaking cystic fibrosis treatments and cutting-edge cancer therapies that help a child's immune system defeat cancer, and made other major contributions to pediatric medicine. 


Researchers work in close collaboration with one another, their colleagues at partner institutions including the University of Washington and Fred Hutch and our healthcare providers at Seattle Children's Hospital, one of U.S. News & World Report’s top children's hospitals. This collaboration is one of our key strengths, allowing our faculty to draw on a variety of disciplines and techniques as they pursue solutions to some of medicine's most complex problems.


We are committed to not only treating disease but to eliminating it. Help us achieve our vision of being a worldwide leader in pediatric research aimed to improve the health and well-being of children. If you are interested in a challenging career aimed at groundbreaking research, Seattle Children's Research Institute is the place for you.


The Immunotherapy Integration Hub (IIH) at Seattle Children’s Research Institute (SCRI) seeks to develop innovative new cellular therapies and translate these advances to groundbreaking clinical trials for children with the most aggressive forms of cancer. We are seeking a bright, enthusiastic, highly-motivated, and experienced molecular and cellular biologist to join the Therapeutic Innovation Core (TIC). This position contributes to a team focused on translating tumor immunotherapy from the bench into pediatric patients with cancer. We look for people who are inspired by our mission and who would fit in well with the collaborative, rigorous, and entrepreneurial spirit of our research team culture. Under the direction of a senior research scientist, the Research Scientist II will utilize established molecular/cellular biology skill sets to create T cell products, develop novel assay platforms to characterize these products and others, and refine and develop manufacturing processes for immunotherapy.


The primary purpose of this position is to plan, design, and execute research development activities in support of funded research projects. The successful candidate will develop and analyze the potential of drug-dependent and cytokine-independent growth of primary T cells. Furthermore, this position will develop and characterize these T cells in the context of CAR-T immunotherapy. Drawing upon established molecular and cellular biology skill sets, this key position will develop methods to create these model and primary T cell lines, develop and perform functional characterization assays, and evaluate the potential for therapeutics via in vivo tumor models.


The ideal candidate will have work experience in molecular biology, tissue culture, immunological assays, and flow cytometry. Responsibilities will include designing experiments; implementing, developing, and optimizing new strategies and assays; generating data; analyzing results; and preparing both written and oral reports in support of funded research studies. To ensure successful project completion and teamwork, the incumbent must have critical thinking abilities, be able to prioritize and perform multiple tasks in a dynamic environment, meet deadlines, and must have excellent written, verbal, and interpersonal communication skills. This position will participate and present data in weekly group meetings and maintain up-to-date and well-organized electronic lab notebook.


Seattle Children's Employer Highlights:
- Leader in Pediatric Care: Because of our people, Seattle Children’s is recognized as a leading teaching, research and specialty care center at the forefront of pediatric care.
- Competitive Benefits: We support a healthy work–life balance. Our benefits include employee care, paid time off, health insurance and retirement savings.
- Transportation Discounts: Company bike program, on-site zip cars, unlimited ORCA transit pass, daily commute bonus provided to staff who us alternate methods of travel to/from work, company vanpools.
- Diversity/Inclusion: We strive to maintain an atmosphere that reflects our values of inclusion by providing effective and respectful care compatible with each patient and family's beliefs, values, and heritage.


Required Education/Experience:

- Bachelor’s Degree in biology, microbiology, immunology, molecular biology, or related fields.

- A minimum of two (2) years’ experience in a related area/laboratory setting.


- Experience culturing adherent, suspension, or primary mammalian cell lines, and isolation and culturing of PBMC and primary human T cells.

- Experience with cell line engineering by transient transfection, electroporation, or viral transduction.

- Experience in multi-color flow cytometry and immunological plate-based assays such as ELISA, signaling activation, cytokine activation, and cytotoxicity assays.

- Experience in assay development, execution, analysis, and troubleshooting.

- Excellent computer skills and extensive working knowledge of Microsoft Office applications, NCBI tools, ELN, FlowJo, GraphPad.

- Ability to effectively prioritize work assignments and address competing demands of multiple projects as part of a collaborative research team.

- Experience working in a Good Laboratory Practice (GLP) regulated environment and understanding of safe lab practices and BSL requirements.

- Significant individual initiative and motivation to conduct research and learn scientific methods and knowledge in respective project area.

Our Commitment to Diversity

Our community welcomes diverse experiences, backgrounds, and thoughts as this is what drives our spirit of inquiry and allows us to better connect with our increasingly diverse patients and families. Our organization recruits, employs, trains, compensates, and promotes without regard to race, religion, color, national origin, gender (including pregnancy, childbirth, or related medical conditions), sexual orientation, gender identity, gender expression, age, status as a protected veteran, status as an individual with a disability, or other applicable legally protected characteristics.


The people who work at Seattle Children’s are members of a community that seeks to respect and celebrate all the qualities that make each of us unique. Each of us is empowered to be ourselves within this community, which cultivates and promotes equity, diversity, and inclusion at all levels.


Seattle Children’s is proud to be an Equal Opportunity Workplace and Affirmative Action Employer.


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