Research Scientist II

Job ID 2020-36161
Area of Interest
Research & Development
Location Name
Jack R MacDonald Building
FTE/Hours per pay period
1.0 FTE (80 hours per bi-weekly pay periods)
Work Status
Immunity & Immunotherapies
Day Shift
Primary Location : City
Primary Location : State/Province

About Us

Hope. Care. Cure. These three simple words capture what we do at Seattle Children’s – to help every child live the healthiest and most fulfilling life possible.  Are you ready to engage with a mission-driven organization that is life-changing to many, and touches the hearts of all? #HOPECARECURE


As one of the nation's top five pediatric research centers, Seattle Children's Research Institute is dedicated to providing hope, care, and cures to help every child live the healthiest and most fulfilling life possible.


Our investigators are involved in hundreds of projects that cover every phase of research, from studying how diseases work to improving investigational therapies. They have pioneered groundbreaking cystic fibrosis treatments and cutting-edge cancer therapies that help a child's immune system defeat cancer, and made other major contributions to pediatric medicine. 


Researchers work in close collaboration with one another, their colleagues at partner institutions including the University of Washington and Fred Hutch and our healthcare providers at Seattle Children's Hospital, one of U.S. News & World Report’s top children's hospitals. This collaboration is one of our key strengths, allowing our faculty to draw on a variety of disciplines and techniques as they pursue solutions to some of medicine's most complex problems.


We are committed to not only treating disease but to eliminating it. Help us achieve our vision of being a worldwide leader in pediatric research aimed to improve the health and well-being of children. If you are interested in a challenging career aimed at groundbreaking research, Seattle Children's Research Institute is the place for you.


The Jackson and Acharya labs (; at Seattle Children’s Research Institute are hiring a motivated scientist to join a rapidly expanding research program on immune responses mediated by B lymphocytes. Our collaborative research group is focused on studying mechanisms that lead to efficient antibody response to viruses and how this is dysregulated during development of autoimmunity. For this position, we are seeking a highly motivated scientist to work with the group in performing experiments related to flow cytometry, ELISA, western blots, B cell gene editing, and tissue culture experiments. Experiments will also involve animal models of disease, include immunisation of mice and harvesting target tissues from mice.

We are looking for an individual who is enthusiastic about taking a hands-on, problem-solving approach, can think analytically, and enjoys working in a collaborative group setting. Most of the responsibilities will be project driven and the candidate is expected to be able to exercise independent judgment in designing and conducting experiments in a rapidly changing, deadline-driven environment. Must be able to interpret, evaluate, analyze, and present data/progress through individual and group meetings as well as accurately document and record experiments in an electronic lab notebook (ELN). Must possess a professional demeanor and excellent verbal, written, interpersonal and organizational skills, as well as the ability to multitask and troubleshoot various issues.


  1. Design experiments to assess B cell responses to activation using flow cytometry and western blot analysis.
  2. Perform in vitro B cell culture and cell activation assays.
  3. Analyze intracellular B cell compartment trafficking by confocal microscopy.
  4. Assist with genotyping and management of mouse colony, and perform experiments using tissues harvested from mice.
  5. Analyse data and present data at group meetings.
  6. Contribute to writing for publication of results; generate figures for papers and grants.


This position would be particularly appropriate for an applicant desiring additional research training prior to applying to graduate or medical school in 2-3 years.


This position is open to either a Res Scientist I or II level contingent upon selected candidate's qualifications and position needs.




Required Education/Experience:

- Bachelor's Degree in an engineering or scientific discipline; may require a Master's Degree for certain lab specialties.

- Minimum to two (2) year's experience in a related area/laboratory setting.



- Master's Degree in applicable field/research specialty highly preferred.

- Significant experience conducting research in area of specialization.

- Strong background in flow cytometry, SDS-PAGE, Western blotting, fluorescence microscopy, PCR and ELISA's.

- Some experience with laboratory management

Our Commitment to Diversity

Our community welcomes diverse experiences, backgrounds, and thoughts as this is what drives our spirit of inquiry and allows us to better connect with our increasingly diverse patients and families. Our organization recruits, employs, trains, compensates, and promotes without regard to race, religion, color, national origin, gender (including pregnancy, childbirth, or related medical conditions), sexual orientation, gender identity, gender expression, age, status as a protected veteran, status as an individual with a disability, or other applicable legally protected characteristics.


The people who work at Seattle Children’s are members of a community that seeks to respect and celebrate all the qualities that make each of us unique. Each of us is empowered to be ourselves within this community, which cultivates and promotes equity, diversity, and inclusion at all levels.


Seattle Children’s is proud to be an Equal Opportunity Workplace and Affirmative Action Employer.


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