Research Scientist II

Job ID 2021-39261
Area of Interest
Research & Development
Location Name
Jack R MacDonald Building
FTE/Hours per pay period
1.0 FTE (80 hours per bi-weekly pay periods)
Work Status
Immunity & Immunotherapies
Day Shift
Primary Location : City
Primary Location : State/Province

About Us

Hope. Care. Cure. These three simple words capture what we do at Seattle Children’s – to help every child live the healthiest and most fulfilling life possible.  Are you ready to engage with a mission-driven organization that is life-changing to many, and touches the hearts of all? #HOPECARECURE


As one of the nation's top five pediatric research centers, Seattle Children's Research Institute is dedicated to providing hope, care, and cures to help every child live the healthiest and most fulfilling life possible.


Our investigators are involved in hundreds of projects that cover every phase of research, from studying how diseases work to improving investigational therapies. They have pioneered groundbreaking cystic fibrosis treatments and cutting-edge cancer therapies that help a child's immune system defeat cancer, and made other major contributions to pediatric medicine. 


Researchers work in close collaboration with one another, their colleagues at partner institutions including the University of Washington and Fred Hutch and our healthcare providers at Seattle Children's Hospital, one of U.S. News & World Report’s top children's hospitals. This collaboration is one of our key strengths, allowing our faculty to draw on a variety of disciplines and techniques as they pursue solutions to some of medicine's most complex problems.


We are committed to not only treating disease but to eliminating it. Help us achieve our vision of being a worldwide leader in pediatric research aimed to improve the health and well-being of children. If you are interested in a challenging career aimed at groundbreaking research, Seattle Children's Research Institute is the place for you.




Principal Investigator:


The Rawlings lab has an open position to work on human primary T cell gene editing and functional analysis for treating immune deficiency diseases.


The successful applicant will work in a fast-paced team environment to develop translational gene editing approaches and cell therapies to restore immune tolerance. Overall responsibilities include:

  1. Work with team lead and members to plan and execute experiments and adhere to project timeline.
  2. Follow detailed experimental protocols and trouble-shoot.
  3. Perform experimental procedures including but not limited to viral vector cloning, cell subtype isolation, CRISPR/Cas9 gene editing, viral transduction, tissue culture, generation of engineered cells, molecular analysis, multi-parameter flow cytometry, ELISA, in vitro functional assays, animal models.
  4. Document observations and data, analyze results, interpret experimental outcomes, maintain detailed and organized electronic data record, and present results in lab meetings.
  5. Assist others in the laboratory and help maintain a constructive laboratory environment. 


This position is ideal for one who is highly motivated and proactive in learning and growing skillsets to prepare for education or career advancement. Great communication, team work, organization, interpersonal and creative thinking skills are a must.


Required Education/Experience:
- Bachelor’s Degree in an engineering or scientific discipline; may require a Master’s Degree for certain lab specialties
- Minimum to two (2) year’s experience in a related area/laboratory setting.

Required Credentials:
- N/A

- Master’s  in biology, microbiology, immunology, molecular biology or related discipline

- 4 years of lab experience, critical thinking and ability to multi-task

Our Commitment to Diversity

Our community welcomes diverse experiences, backgrounds, and thoughts as this is what drives our spirit of inquiry and allows us to better connect with our increasingly diverse patients and families. Our organization recruits, employs, trains, compensates, and promotes without regard to race, religion, color, national origin, gender (including pregnancy, childbirth, or related medical conditions), sexual orientation, gender identity, gender expression, age, status as a protected veteran, status as an individual with a disability, or other applicable legally protected characteristics.


The people who work at Seattle Children’s are members of a community that seeks to respect and celebrate all the qualities that make each of us unique. Each of us is empowered to be ourselves within this community, which cultivates and promotes equity, diversity, and inclusion at all levels.


Seattle Children’s is proud to be an Equal Opportunity Workplace and Affirmative Action Employer.


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