Research Scientist II

Job ID 2021-39599
Area of Interest
Research & Development
Location Name
1920 Terry
FTE/Hours per pay period
1.0 FTE (80 hours per bi-weekly pay periods)
Work Status
Regular
Department
SC Therapeutics (SCTx)
Shift
Day Shift
Primary Location : City
Seattle
Primary Location : State/Province
WA

About Us

Hope. Care. Cure. These three simple words capture what we do at Seattle Children’s – to help every child live the healthiest and most fulfilling life possible.  Are you ready to engage with a mission-driven organization that is life-changing to many, and touches the hearts of all? #HOPECARECURE

 

As one of the nation's top five pediatric research centers, Seattle Children's Research Institute is dedicated to providing hope, care, and cures to help every child live the healthiest and most fulfilling life possible.

 

Our investigators are involved in hundreds of projects that cover every phase of research, from studying how diseases work to improving investigational therapies. They have pioneered groundbreaking cystic fibrosis treatments and cutting-edge cancer therapies that help a child's immune system defeat cancer, and made other major contributions to pediatric medicine. 

 

Researchers work in close collaboration with one another, their colleagues at partner institutions including the University of Washington and Fred Hutch and our healthcare providers at Seattle Children's Hospital, one of U.S. News & World Report’s top children's hospitals. This collaboration is one of our key strengths, allowing our faculty to draw on a variety of disciplines and techniques as they pursue solutions to some of medicine's most complex problems.

 

We are committed to not only treating disease but to eliminating it. Help us achieve our vision of being a worldwide leader in pediatric research aimed to improve the health and well-being of children. If you are interested in a challenging career aimed at groundbreaking research, Seattle Children's Research Institute is the place for you.

Overview

This position will contribute to a team focused on translating tumor immunotherapy from the bench to the beside of pediatric cancer patients. The primary purpose of this position is to aid in the effort of discovering chimeric antigen receptor (CAR)-modifying transgenes that will enhance cell therapies for solid tumors, a process that involves developing and analyzing vectors carrying regulated transgene payloads. Under the direction of a senior research scientist, the successful candidate will use their established molecular and cellular biology skill sets to create both tumor and primary T cell lines and develop and implement assays to determine the cytotoxic and therapeutic potential of the T cell products, both in vitro and in vivo.  Lab techniques include basic and advanced molecular biology, primary T cell culture and operation of Incucyte, MSD, flow cytometers, cell sorters and liquid handling robots.

This position requires significant work experience in flow cytometry, tissue culture, molecular biology, and immunology. The ideal candidate will have experience in independent experimental design, execution, analysis, and presentation.  We are looking for a candidate who has strong critical thinking abilities, the ability to prioritize and perform multiple tasks in a dynamic, and fast-paced environment. Importantly, this is a collaborative environment that demands excellent written, verbal, and interpersonal communication skills for success. To that end, the candidate will contribute to weekly lab meetings, maintain an up-to-date and well-organized electronic lab notebook, and rigorously follow Good Laboratory Practice (GLP) guidelines.  Above all, a willingness to learn, passion for our mission, collaboration, and creativity are attributes that we feel are crucial for helping Seattle Children's Therapeutics (SCTx) create novel and robust cures for our patients

 

Responsible for independently evaluating, selecting and applying standardized scientific procedures and techniques; contributes to the planning, design, execution and interpretation of experiments; makes detailed observations, records data and assists in preparation of reports, papers presentations, and new proposals; presents results/papers at technical meetings; may participate in the guidance/mentoring of junior staff.

Requirements

Required Experience:

- demonstrated experience with multiparameter flow cytometry, including panel design, analysis and reporting

- experience with aseptic technique, human primary cell culture and cryopreservation

- ability to work in a dynamic, collaborative setting

- excellent oral and written communication skills

 

Preferred Experience:

- basic and advanced molecular biology including cloning, PCR, qPCR, and ddPCR

- experiences in nonviral vector delivery systems

- primary T cell culture and operation of Incucyte and MSD

- FACS, HTS flow cytometry and liquid handling robots

 

Required Education:

- B.S. in molecular biology, cell biology, microbiology, immunology or related scientific discipline

- Minimum two (2) years’ experience in a related area/laboratory setting


Required Credentials:
- N/A

 

Preferred Education:

- Master’s degree in molecular biology, cell biology, microbiology, immunology or related scientific discipline

- Strong foundation conducting research related to CAR-T or T cell biology

 

In accordance with new Washington law for healthcare workers, Seattle Children’s requires that all staff be fully vaccinated against COVID-19 on or before October 18, 2021, or before beginning employment for start dates after October 18th.

Our Commitment to Diversity

Our community welcomes diverse experiences, backgrounds, and thoughts as this is what drives our spirit of inquiry and allows us to better connect with our increasingly diverse patients and families. Our organization recruits, employs, trains, compensates, and promotes without regard to race, religion, color, national origin, gender (including pregnancy, childbirth, or related medical conditions), sexual orientation, gender identity, gender expression, age, status as a protected veteran, status as an individual with a disability, or other applicable legally protected characteristics.

 

The people who work at Seattle Children’s are members of a community that seeks to respect and celebrate all the qualities that make each of us unique. Each of us is empowered to be ourselves within this community, which cultivates and promotes equity, diversity, and inclusion at all levels.

 

Seattle Children’s is proud to be an Equal Opportunity Workplace and Affirmative Action Employer.

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