Research Technician II (Infections & Prematurity)

Job ID 2021-39739
Area of Interest
Research & Development
Location Name
307 Westlake
FTE/Hours per pay period
1.0 FTE (80 hours per bi-weekly pay periods)
Work Status
Global Infectious Disease
Day Shift
Primary Location : City
Primary Location : State/Province

About Us

Hope. Care. Cure. These three simple words capture what we do at Seattle Children’s – to help every child live the healthiest and most fulfilling life possible.  Are you ready to engage with a mission-driven organization that is life-changing to many, and touches the hearts of all? #HOPECARECURE


As one of the nation's top five pediatric research centers, Seattle Children's Research Institute is dedicated to providing hope, care, and cures to help every child live the healthiest and most fulfilling life possible.


Our investigators are involved in hundreds of projects that cover every phase of research, from studying how diseases work to improving investigational therapies. They have pioneered groundbreaking cystic fibrosis treatments and cutting-edge cancer therapies that help a child's immune system defeat cancer, and made other major contributions to pediatric medicine. 


Researchers work in close collaboration with one another, their colleagues at partner institutions including the University of Washington and Fred Hutch and our healthcare providers at Seattle Children's Hospital, one of U.S. News & World Report’s top children's hospitals. This collaboration is one of our key strengths, allowing our faculty to draw on a variety of disciplines and techniques as they pursue solutions to some of medicine's most complex problems.


We are committed to not only treating disease but to eliminating it. Help us achieve our vision of being a worldwide leader in pediatric research aimed to improve the health and well-being of children. If you are interested in a challenging career aimed at groundbreaking research, Seattle Children's Research Institute is the place for you.


The Coler Preclinical & Translational Research Group is looking for talented and dedicated technicians to work with animal models and perform a wide array of microbiological and immunological assays to develop vaccines against diseases caused by Mycobacterium tuberculosis, nontuberculous mycobacteria and positive strand RNA viruses e.g., SARS-CoV-2. The successful candidate will be committed to SCRI’s mission of developing vaccines for infectious diseases, be a strong team player capable of working with moderate supervision and committed to learning new skills. A strong background in microbiology is preferred. Major techniques include immunology assays, cell culture, and animal work.



• Animal work including injections via multiple routes, blood collection, dissections, tissue collection, monitoring temperatures, weights in mice under BSL2/BSL3 conditions

• Perform intracellular cytokine staining/multi-color flow cytometry, antibody neutralization, ELISPOTS, ELISA and other immunological assays

• Infecting mice under supervision and BSL2/3 conditions

• Enumeration of positive strand RNA viruses, nontuberculous mycobacteria and/or Mycobacterium tuberculosis from experimental animals by culture under BSL3 conditions

• Prepare blood and tissues for ex vivo assays under BSL2/BSL3 conditions

• Monitors laboratory supply inventories and works with purchasing to ensure available stocks

• Communicates with research management on a regular basis 

• Utilize mathematical and scientific techniques to compile and analyze data

• Maintain the highest standards of laboratory practice in line with IDRI policy

• Strong organizational and mathematical skills (e.g. dilutions, conversions, overage calculations)

• Close attention to detail including recording observations and findings in audited lab notebooks following established guidelines

• Excellent oral and written communication skills

• Proficiency in MS Office and scientific data/graphics/statistical programs 

• Proficient in trouble-shooting experiments and maintenance of equipment 

• Ability to multi-task and perform multiple scientific protocols and procedures under direction  

• Ability to maintain confidentiality of sensitive information

• Takes initiative to schedule work and has excellent time management skills

• Possess a work ethic that includes neatness, punctuality, and accuracy

• Must be self-motivated, organized and be able to work in a team environment

• Enjoys learning new concepts or technical skills 

• Does not require travel outside of Seattle

• Requires infrequent evening or weekend work


Required Qualifications: 

• A BSc/BA, or equivalent in Microbiology, Immunology, Molecular and Cellular Biology 

• 1-3 years full-time laboratory experience in microbiology or immunology lab employment beyond academic courses

• Excellent oral and written communication skills.

• Computer literacy, proficiency in MS Office programs

• Experience and ability to work with small animal models of infectious disease required

• Knowledge of immunology – coursework or lab work

• Experience working under ABSL2 or ABSL3 conditions

• Bacteriology and/or virology experience; interest in infectious disease

• Although these qualifications are desired, we are particularly interested in training the next generation of scientists. Those who are seeking laboratory experience as a stepping stone to becoming a scientist and were unable to do so while doing undergraduate school are encouraged to apply.

Our Commitment to Diversity

Our community welcomes diverse experiences, backgrounds, and thoughts as this is what drives our spirit of inquiry and allows us to better connect with our increasingly diverse patients and families. Our organization recruits, employs, trains, compensates, and promotes without regard to race, religion, color, national origin, gender (including pregnancy, childbirth, or related medical conditions), sexual orientation, gender identity, gender expression, age, status as a protected veteran, status as an individual with a disability, or other applicable legally protected characteristics.


The people who work at Seattle Children’s are members of a community that seeks to respect and celebrate all the qualities that make each of us unique. Each of us is empowered to be ourselves within this community, which cultivates and promotes equity, diversity, and inclusion at all levels.


Seattle Children’s is proud to be an Equal Opportunity Workplace and Affirmative Action Employer.


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