Fellow - PhD, Research – Viral-Host Cell Interactions

Job ID 2021-40235
Area of Interest
Research & Development
Location Name
Jack R MacDonald Building
FTE/Hours per pay period
1.0 FTE (80 hours per bi-weekly pay periods)
Work Status
Regular
Department
Immunity & Immunotherapies
Shift
Day Shift
Primary Location : City
Seattle
Primary Location : State/Province
WA

Overview

About Torbett Lab and projects

 

The laboratory of Dr. Bruce E. Torbett (www.torbettlab.org) is looking for a Senior Post Doctoral Fellow to work on projects related to the Structural and Biophysical Interactions of HIV Gag/Gag-Pol polyproteins with their host cell proteins during viral trafficking and assembly. This work aims to investigate how HIV co-opts cellular host machinery during active infection and identify the composition, dynamics, and function of novel host-viral interfaces that can be modulated for new therapeutic strategies. This work involves studies utilizing proximity labeling and quantitative proteomics, along with biochemical and virological validation of candidate cellular factors using CRISPR/Cas9 knockouts and confocal imaging techniques. Candidates must have a Ph.D. in Biochemistry or related field with documented experience (work or academic) in Mass Spectrometry-based Proteomics to identify host-factors of microbial infections, along with experience in target validation using relevant in vivo and in vitro assays.

 

The group works as a highly dynamic and interactive team and is part of the Center for Immunity and Immunotherapies (CIIT) of Seattle Children’s Research Institute (SCRI). Our studies are funded through a number of grants from the NIH (including the flagship HIVE Center). You will have the opportunity to interact and form collaborations with CIIT, SCRI and HIVE Center Investigators, as well as with collaborators throughout the Seattle area (University of Washington, Fred Hutch)

 

Seattle Children’s Research Institute and Torbett Lab offer competitive salaries,  relocation assistance (if/as applicable), in addition to eligibility to a competitive benefits package as a full time employee

 

About the Position

    • Develop scientific skills as appropriate for this training position as a postdoctoral fellow
    • Meet with Principal Investigator on a regular basis to discuss proposed/ongoing studies, discuss implications of findings, and provide insights into new research directions
    • Run research projects in an independent manner with oversight from Principal Investigator
    • Write manuscripts, present data at national and international conferences
    • Assist PI in writing progress reports, grant proposals, protocols as relevant to the postdoctoral fellow training
    • Protocol development and review
    • Excellent communication and writing skills
    • Develop additional scientific skills as appropriate and carry out research/development activities of assigned research projects. 
    • Ability to learn and apply new technologies and skills

Requirements

Qualifications

 

Ph.D. in Biochemistry or related field

 

Related work experience must include the following:

- Experience in Mass Spectrometry-based Proteomics to identify host-factors of microbial infections

- Experience in target validation using relevant in vivo and in vitro assays (eg. Gene targeting with CRISPR/Cas9)

- Cell culture (adherent/suspension cell lines)

- Fluorescence Microscopy

- Various methods of protein isolation, labelling, qualification, and quantification

- Bioconjugation and/or chemical biology

 

Preferred Qualifications:

- Working knowledge of high-throughput screening technologies

- Immunoassays (such as p24 ELISA)

- Knowledge of Structural Biology

- Confocal Microscopy

- Knowledge of Bioinformatics as it pertains to analysis of proteomics datasets

- Protein Chemistry and Chemical Biology

- Protein engineering

 

 

About Us

 

Hope. Care. Cure. These three simple words capture what we do at Seattle Children’s – to help every child live the healthiest and most fulfilling life possible.  Are you ready to engage with a mission-driven organization that is life-changing to many, and touches the hearts of all? #HOPECARECURE

 

As one of the nation's top five pediatric research centers, Seattle Children's Research Institute is dedicated to providing hope, care, and cures to help every child live the healthiest and most fulfilling life possible.

 

Our investigators are involved in hundreds of projects that cover every phase of research, from studying how diseases work to improving investigational therapies. They have pioneered groundbreaking cystic fibrosis treatments and cutting-edge cancer therapies that help a child's immune system defeat cancer, and made other major contributions to pediatric medicine. 

 

Researchers work in close collaboration with one another, their colleagues at partner institutions including the University of Washington and Fred Hutch and our healthcare providers at Seattle Children's Hospital, one of U.S. News & World Report’s top children's hospitals. This collaboration is one of our key strengths, allowing our faculty to draw on a variety of disciplines and techniques as they pursue solutions to some of medicine's most complex problems.

 

We are committed to not only treating disease but to eliminating it. Help us achieve our vision of being a worldwide leader in pediatric research aimed to improve the health and well-being of children. If you are interested in a challenging career aimed at groundbreaking research, Seattle Children's Research Institute is the place for you.

 

 

In accordance with new Washington law for healthcare workers, Seattle Children’s requires that all staff be fully vaccinated against COVID-19 on or before October 18, 2021, or before beginning employment for start dates after October 18th.

Covid-19 Vaccination

In accordance with Washington state law, Seattle Children’s requires that all employees be fully vaccinated against COVID-19. All offers are contingent and your vaccine status will be verified at onboarding.

Our Commitment to Diversity

Our community welcomes diverse experiences, backgrounds, and thoughts as this is what drives our spirit of inquiry and allows us to better connect with our increasingly diverse patients and families. Our organization recruits, employs, trains, compensates, and promotes without regard to race, religion, color, national origin, gender (including pregnancy, childbirth, or related medical conditions), sexual orientation, gender identity, gender expression, age, status as a protected veteran, status as an individual with a disability, or other applicable legally protected characteristics.

 

The people who work at Seattle Children’s are members of a community that seeks to respect and celebrate all the qualities that make each of us unique. Each of us is empowered to be ourselves within this community, which cultivates and promotes equity, diversity, and inclusion at all levels.

 

Seattle Children’s is proud to be an Equal Opportunity Workplace and Affirmative Action Employer.

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