About Torbett Lab and projects
The laboratory of Dr. Bruce E. Torbett (www.torbettlab.org) is looking for a Senior Post Doctoral Fellow to work on projects related to the Structural and Biophysical Interactions of HIV Gag/Gag-Pol polyproteins with their host cell proteins during viral trafficking and assembly. This work aims to investigate how HIV co-opts cellular host machinery during active infection and identify the composition, dynamics, and function of novel host-viral interfaces that can be modulated for new therapeutic strategies. This work involves studies utilizing proximity labeling and quantitative proteomics, along with biochemical and virological validation of candidate cellular factors using CRISPR/Cas9 knockouts and confocal imaging techniques. Candidates must have a Ph.D. in Biochemistry or related field with documented experience (work or academic) in Mass Spectrometry-based Proteomics to identify host-factors of microbial infections, along with experience in target validation using relevant in vivo and in vitro assays.
The group works as a highly dynamic and interactive team and is part of the Center for Immunity and Immunotherapies (CIIT) of Seattle Children’s Research Institute (SCRI). Our studies are funded through a number of grants from the NIH (including the flagship HIVE Center). You will have the opportunity to interact and form collaborations with CIIT, SCRI and HIVE Center Investigators, as well as with collaborators throughout the Seattle area (University of Washington, Fred Hutch)
Seattle Children’s Research Institute and Torbett Lab offer competitive salaries, relocation assistance (if/as applicable), in addition to eligibility to a competitive benefits package as a full time employee
About the Position
Ph.D. in Biochemistry or related field
Related work experience must include the following:
- Experience in Mass Spectrometry-based Proteomics to identify host-factors of microbial infections
- Experience in target validation using relevant in vivo and in vitro assays (eg. Gene targeting with CRISPR/Cas9)
- Cell culture (adherent/suspension cell lines)
- Fluorescence Microscopy
- Various methods of protein isolation, labelling, qualification, and quantification
- Bioconjugation and/or chemical biology
- Working knowledge of high-throughput screening technologies
- Immunoassays (such as p24 ELISA)
- Knowledge of Structural Biology
- Confocal Microscopy
- Knowledge of Bioinformatics as it pertains to analysis of proteomics datasets
- Protein Chemistry and Chemical Biology
- Protein engineering
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