Perform laboratory procedures and experiments investigating the role of the airway remodeling and the extracellular matrix (ECM) in lung disease during acute and chronic airway inflammation using ex vivo primary human cell culture models. Technical skills include human primary airway epithelial cell, lung stromal cell, and immune cell tissue culture, differentiation of airway epithelial cells at an air-liquid interface, gene knockdown using lentiviral shRNA and/or gene editing using CRISPR/Cas9 methods in primary human cell lines, microscopy (phase contrast; fluorescent), biochemical assays, RNA isolation, qPCR, protein assays and ELISA.
Work includes infection of airway epithelial cells in a BSL-2 laboratory with respiratory viruses. Work as part of a small lab with close collaboration and support of a larger group of lab technicians, students, clinical research associates, and principal investigators.
Complete daily lab tasks including design and execution of experiments, maintenance of laboratory records, and opportunities for authorship on manuscripts.
- Bachelor’s Degree in an engineering or scientific discipline
- Prior experience in a laboratory setting; may include relevant educational programs.
At least one (1) year of experience in the following:
- Set up and maintenance of tissue culture; ideally primary cell culture
- RNA isolation, cDNA synthesis, quantitative PCR using TaqMan probes
- Conduct of protein and ELISA assays
- Use of shRNA or siRNA for gene knockdown experiments and/or CRISPR-Cas9 gene editing in primary cells
- Flow cytometry
- Experience with phase contrast and fluorescent microscopy
- Data entry and/or word processing experience
- Experience in analyzing data using spreadsheets and statistical analysis.
Hope. Care. Cure. These three simple words capture what we do at Seattle Children’s – to help every child live the healthiest and most fulfilling life possible. Are you ready to engage with a mission-driven organization that is life-changing to many, and touches the hearts of all? #HOPECARECURE
As one of the nation's top five pediatric research centers, Seattle Children's Research Institute is dedicated to providing hope, care, and cures to help every child live the healthiest and most fulfilling life possible.
Our investigators are involved in hundreds of projects that cover every phase of research, from studying how diseases work to improving investigational therapies. They have pioneered groundbreaking cystic fibrosis treatments and cutting-edge cancer therapies that help a child's immune system defeat cancer, and made other major contributions to pediatric medicine.
Researchers work in close collaboration with one another, their colleagues at partner institutions including the University of Washington and Fred Hutch and our healthcare providers at Seattle Children's Hospital, one of U.S. News & World Report’s top children's hospitals. This collaboration is one of our key strengths, allowing our faculty to draw on a variety of disciplines and techniques as they pursue solutions to some of medicine's most complex problems.
We are committed to not only treating disease but to eliminating it. Help us achieve our vision of being a worldwide leader in pediatric research aimed to improve the health and well-being of children. If you are interested in a challenging career aimed at groundbreaking research, Seattle Children's Research Institute is the place for you.
Our community welcomes diverse experiences, backgrounds, and thoughts as this is what drives our spirit of inquiry and allows us to better connect with our increasingly diverse patients and families. Our organization recruits, employs, trains, compensates, and promotes without regard to race, religion, color, national origin, gender (including pregnancy, childbirth, or related medical conditions), sexual orientation, gender identity, gender expression, age, status as a protected veteran, status as an individual with a disability, or other applicable legally protected characteristics.
The people who work at Seattle Children’s are members of a community that seeks to respect and celebrate all the qualities that make each of us unique. Each of us is empowered to be ourselves within this community, which cultivates and promotes equity, diversity, and inclusion at all levels.
Seattle Children’s is proud to be an Equal Opportunity Workplace and Affirmative Action Employer.